ABSTRACT
Abstract The aim of this study was to evaluate whether the modification in the silver component is capable of providing GuttaFlow 2 with antibacterial activity against Enterococcus faecalis compared with epoxy resin-based (AH Plus) and zinc oxide and eugenol-based (Endofill) sealers. The antibacterial activity was evaluated using a reference strain of E. faecalis (ATCC 29212). Freshly mixed sealers were subjected to the agar diffusion test (ADT), while the direct contact test (DCT) was performed after materials setting. ADT results were obtained through measurements, in millimeters, of the inhibition zones promoted by the materials, using a digital caliper. In DCT, values of CFU/mL promoted by the three sealers were compared in three experimental periods (1 min, 1 h, and 24 h). The data were analyzed using Kruskal-Wallis and Dunn post-hoc tests (p < 0.05). In both ADT and DCT, GuttaFlow 2 presented no effect against E. faecalis, while Endofill and AH Plus showed similar inhibition zones. Endofill was the only material capable of reducing bacterial growth in DCT. In conclusion, modifications in the silver particle of GuttaFlow 2 did not result in a sealer with antibacterial effect against E. faecalis.
Subject(s)
Root Canal Filling Materials/pharmacology , Silver/pharmacology , Enterococcus faecalis/drug effects , Dimethylpolysiloxanes/pharmacology , Epoxy Resins/pharmacology , Gutta-Percha/pharmacology , Anti-Bacterial Agents/pharmacology , Particle Size , Root Canal Filling Materials/chemistry , Silicones/pharmacology , Silicones/chemistry , Silver/chemistry , Time Factors , Materials Testing , Colony Count, Microbial , Reproducibility of Results , Statistics, Nonparametric , Dimethylpolysiloxanes/chemistry , Drug Combinations , Epoxy Resins/chemistry , Gutta-Percha/chemistry , Anti-Bacterial Agents/chemistryABSTRACT
Todas nós fomos formadas durante a embriogênese. Algumas de nós, as escolhidas, vieram do epitélio bucal e outras, do ectomesênquima (tecido de origem neural). Me refiro às escolhidas por que, ainda na fase intrauterina do ser humano que eu costumava habitar, elas receberam o estímulo necessário para participar do espetáculo clássico intitulado A formação dentária, e se diferenciaram em lindas células ameloblásticas, odontoblásticas, cementoblásticas ou osteoblásticas. Cada uma tinha uma função específica, e algumas delas exercem essa função até hoje.(AU) [...]